UNITED STATES
                       SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                    Form 13F
                               Form 13F COVER PAGE
Report for the Calendar Year or Quarter Ended: 9/30/2009
Check here if Amendment []; Amendment Number:
                                               -------
This Amendment (Check only one.):               [] is a restatement.
                                                [] adds new holdings entries.

Institutional Investment Manager Filing this Report:


Name:      Burke & Herbert Bank & Trust Co.

Address:   PO Box 268 Alexandria, VA 22314


Form 13F File Number: 28-05313


The institutional investment manager filing this report and the person by whom
it is signed hereby represent that the person signing the report is authorized
to submit it, that all information contained herein is true, correct and
complete, and that it is understood that all required items, statements,
schedules, lists, and tables, are considered integral parts of this form.

Persons Signing this Report on Behalf of Reporting Manager:

Name:   Judith A. Cagnon
Title:  Vice President
Phone:  703-549-6600

Signature,  Place,  and  Date  of  Signing:

/s/ Judith A. Cagnon               Alexandria, VA                     10/5/2009
---------------------------------  ---------------------------------  ----------
[Signature]                        [City, State]                      [Date]



Report  Type  (Check  only  one):

[X]     13F HOLDINGS REPORT. (Check here if all holdings of this reporting
        manager are reported in this report.)

[ ]     13F NOTICE. (Check here if no holdings reported are in this report,
        and all holdings are reported by other reporting manager(s).)

[ ]     13F COMBINATION REPORT. (Check here if a portion of the holdings for
        this reporting manager are reported in this report and a portion are
        reported by other reporting manager(s).)

List of Other Managers Reporting for this Manager:




                              Form 13F SUMMARY PAGE

Report Summary:

Number of Other Included Managers:                    0

Form 13F Information Table Entry Total:             165

Form 13F Information Table Value Total:  $    79,654.00
                                         --------------
                                         (In Thousands)


List of Other Included Managers:

Provide a numbered list of the name(s) and 13F file number(s) of all
institutional investment managers with respect to which this report is filed,
other than the manager filing this report.
NONE







                                                  FORM 13F INFORMATION TABLE

            COLUMN 1                  COLUMN 2      COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
--------------------------------- ----------------- --------- -------- ----------------- ---------- -------- -------------------
                                                               VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
         NAME OF ISSUER            TITLE OF CLASS     CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
--------------------------------- ----------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                           
INGERSOLL-RAND PLC                SHS               G47791101      273    8,900 SH       DEFINED               8,900      0    0
TRANSOCEAN LTD                    REG SHS           H8817H100       32      369 SH       SOLE                    369      0    0
TRANSOCEAN LTD                    REG SHS           H8817H100      309    3,613 SH       DEFINED               3,613      0    0
AT&T INC                          COM               00206R102      771   28,528 SH       SOLE                 28,528      0    0
AT&T INC                          COM               00206R102      758   28,061 SH       DEFINED              28,061      0    0
ABBOTT LABS                       COM               002824100      574   11,600 SH       SOLE                 11,600      0    0
ABBOTT LABS                       COM               002824100      409    8,260 SH       DEFINED               8,260      0    0
AIR PRODS & CHEMS INC             COM               009158106       23      300 SH       SOLE                    300      0    0
AIR PRODS & CHEMS INC             COM               009158106      182    2,350 SH       DEFINED               2,350      0    0
ALLIED CAP CORP NEW               COM               01903Q108        0       25 SH       SOLE                     25      0    0
ALLIED CAP CORP NEW               COM               01903Q108       42   13,675 SH       DEFINED              13,675      0    0
ALTRIA GROUP INC                  COM               02209S103      351   19,700 SH       SOLE                 19,700      0    0
ALTRIA GROUP INC                  COM               02209S103      103    5,800 SH       DEFINED               5,800      0    0
AMERICAN EXPRESS CO               COM               025816109      168    4,966 SH       SOLE                  4,966      0    0
AMERICAN EXPRESS CO               COM               025816109       42    1,250 SH       DEFINED               1,250      0    0
APPLE INC                         COM               037833100      133      720 SH       SOLE                    720      0    0
APPLE INC                         COM               037833100      310    1,675 SH       DEFINED               1,675      0    0
AUTOMATIC DATA PROCESSING IN      COM               053015103       12      300 SH       SOLE                    300      0    0
AUTOMATIC DATA PROCESSING IN      COM               053015103      259    6,600 SH       DEFINED               6,600      0    0
BP PLC                            SPON ADR          055622104      763   14,332 SH       SOLE                 14,332      0    0
BP PLC                            SPON ADR          055622104      261    4,897 SH       DEFINED               4,897      0    0
BANCO BILBAO VIZCAYA ARGENTA      SPON ADR          05946K101      251   14,045 SH       SOLE                 14,045      0    0
BANK OF AMERICA CORPORATION       COM               060505104      125    7,408 SH       SOLE                  7,408      0    0
BANK OF AMERICA CORPORATION       COM               060505104      612   36,196 SH       DEFINED              36,196      0    0
BANK OF NEW YORK MELLON CORP      COM               064058100        4      150 SH       SOLE                    150      0    0
BANK OF NEW YORK MELLON CORP      COM               064058100      439   15,139 SH       DEFINED              15,139      0    0
BAXTER INTL INC                   COM               071813109      191    3,350 SH       SOLE                  3,350      0    0
BAXTER INTL INC                   COM               071813109       43      750 SH       DEFINED                 750      0    0
BERKSHIRE HATHAWAY INC DEL        CL A              084670108      303        3 SH       SOLE                      3      0    0
BLACKROCK INC                     COM               09247X101       92      425 SH       SOLE                    425      0    0
BLACKROCK INC                     COM               09247X101      184      850 SH       DEFINED                 850      0    0
BOEING CO                         COM               097023105      169    3,125 SH       SOLE                  3,125      0    0
BOEING CO                         COM               097023105       43      800 SH       DEFINED                 500     30    0
BRISTOL MYERS SQUIBB CO           COM               110122108      224    9,926 SH       SOLE                  9,926      0    0
BRISTOL MYERS SQUIBB CO           COM               110122108      136    6,020 SH       DEFINED               6,020      0    0
BURKE&HERBERT B&T ALEXANDRIA      COM               121331102   11,377    6,742 SH       SOLE                  6,742      0    0
BURKE&HERBERT B&T ALEXANDRIA      COM               121331102    1,382      819 SH       DEFINED                 819      0    0
BURLINGTON NORTHN SANTA FE C      COM               12189T104       22      279 SH       SOLE                    279      0    0
BURLINGTON NORTHN SANTA FE C      COM               12189T104      322    4,038 SH       DEFINED               4,038      0    0
CIGNA CORP                        COM               125509109      426   15,180 SH       DEFINED              15,180      0    0
CVS CAREMARK CORPORATION          COM               126650100      307    8,600 SH       DEFINED               8,600      0    0
CHEVRON CORP NEW                  COM               166764100      757   10,749 SH       SOLE                 10,749      0    0
CHEVRON CORP NEW                  COM               166764100    1,498   21,271 SH       DEFINED              21,271      0    0
CISCO SYS INC                     COM               17275R102      330   14,024 SH       SOLE                 14,024      0    0
CISCO SYS INC                     COM               17275R102      268   11,400 SH       DEFINED              11,400      0    0
CITIGROUP INC                     COM               172967101       14    2,903 SH       SOLE                  2,903      0    0
CITIGROUP INC                     COM               172967101      453   93,695 SH       DEFINED              93,695      0    0
COCA COLA CO                      COM               191216100      630   11,730 SH       SOLE                 11,730      0    0
COCA COLA CO                      COM               191216100      507    9,450 SH       DEFINED               9,450      0    0
COLGATE PALMOLIVE CO              COM               194162103      251    3,295 SH       SOLE                  3,295      0    0
COLGATE PALMOLIVE CO              COM               194162103      149    1,950 SH       DEFINED               1,950      0    0
CONOCOPHILLIPS                    COM               20825C104       26      580 SH       SOLE                    580      0    0
CONOCOPHILLIPS                    COM               20825C104      175    3,880 SH       DEFINED               3,880      0    0
CONSOLIDATED EDISON INC           COM               209115104       85    2,071 SH       SOLE                  2,071      0    0
CONSOLIDATED EDISON INC           COM               209115104      147    3,579 SH       DEFINED               3,579      0    0
DELL INC                          COM               24702R101       66    4,350 SH       SOLE                  4,350      0    0
DELL INC                          COM               24702R101      148    9,700 SH       DEFINED               9,700      0    0
DISNEY WALT CO                    COM               254687106      211    7,700 SH       SOLE                  7,700      0    0
DISNEY WALT CO                    COM               254687106      394   14,350 SH       DEFINED              14,350      0    0
DOMINION RES INC VA NEW           COM               25746U109      367   10,642 SH       SOLE                 10,642      0    0






                                                  FORM 13F INFORMATION TABLE

            COLUMN 1                  COLUMN 2      COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
--------------------------------- ----------------- --------- -------- ----------------- ---------- -------- -------------------
                                                               VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
         NAME OF ISSUER            TITLE OF CLASS     CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
--------------------------------- ----------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                           
DOMINION RES INC VA NEW           COM               25746U109      346   10,024 SH       DEFINED              10,024      0    0
DU PONT E I DE NEMOURS & CO       COM               263534109      414   12,872 SH       SOLE                 12,872      0    0
DU PONT E I DE NEMOURS & CO       COM               263534109      538   16,726 SH       DEFINED              16,726      0    0
DUKE ENERGY CORP NEW              COM               26441C105       28    1,763 SH       SOLE                  1,763      0    0
DUKE ENERGY CORP NEW              COM               26441C105      154    9,800 SH       DEFINED               9,800      0    0
DUN & BRADSTREET CORP DEL NE      COM               26483E100      271    3,600 SH       DEFINED               3,600      0    0
EMERSON ELEC CO                   COM               291011104      135    3,364 SH       SOLE                  3,364      0    0
EMERSON ELEC CO                   COM               291011104       95    2,360 SH       DEFINED               2,360      0    0
EXELON CORP                       COM               30161N101      143    2,882 SH       SOLE                  2,882      0    0
EXELON CORP                       COM               30161N101      189    3,800 SH       DEFINED               3,800      0    0
EXXON MOBIL CORP                  COM               30231G102    2,999   43,709 SH       SOLE                 43,709      0    0
EXXON MOBIL CORP                  COM               30231G102    4,596   66,986 SH       DEFINED              66,986      0    0
FPL GROUP INC                     COM               302571104      278    5,032 SH       SOLE                  5,032      0    0
FPL GROUP INC                     COM               302571104      165    2,990 SH       DEFINED               2,990      0    0
FEDERAL REALTY INVT TR SH BEN INT NEW               313747206      562    9,150 SH       DEFINED               9,150      0    0
FLUOR CORP NEW                    COM               343412102      315    6,200 SH       DEFINED               6,200      0    0
FORTUNE BRANDS INC                COM               349631101      322    7,500 SH       DEFINED               7,500      0    0
FREEPORT-MCMORAN COPPER & GO      COM               35671D857      784   11,421 SH       DEFINED              11,421      0    0
GENERAL ELECTRIC CO               COM               369604103    1,754  106,791 SH       SOLE                106,791      0    0
GENERAL ELECTRIC CO               COM               369604103    1,922  117,023 SH       DEFINED             117,023      0    0
GOLDMAN SACHS GROUP INC           COM               38141G104       72      390 SH       SOLE                    390      0    0
GOLDMAN SACHS GROUP INC           COM               38141G104      144      780 SH       DEFINED                 780      0    0
HEWLETT PACKARD CO                COM               428236103      983   20,812 SH       SOLE                 20,812      0    0
HEWLETT PACKARD CO                COM               428236103      861   18,245 SH       DEFINED              18,245      0    0
HOME DEPOT INC                    COM               437076102      127    4,775 SH       SOLE                  4,775      0    0
HOME DEPOT INC                    COM               437076102      151    5,683 SH       DEFINED               5,683      0    0
HONEYWELL INTL INC                COM               438516106      195    5,262 SH       SOLE                  5,262      0    0
HONEYWELL INTL INC                COM               438516106      186    5,000 SH       DEFINED               5,000      0    0
INTEL CORP                        COM               458140100      328   16,778 SH       SOLE                 16,778      0    0
INTEL CORP                        COM               458140100      179    9,125 SH       DEFINED               8,325     80    0
INTERNATIONAL BUSINESS MACHS      COM               459200101      647    5,412 SH       SOLE                  5,412      0    0
INTERNATIONAL BUSINESS MACHS      COM               459200101      510    4,262 SH       DEFINED               4,262      0    0
ISHARES TR INDEX BARCLY           USAGG B           464287226      248    2,365 SH       SOLE                  2,365      0    0
JPMORGAN CHASE & CO               COM               46625H100      178    4,055 SH       SOLE                  4,055      0    0
JPMORGAN CHASE & CO               COM               46625H100       34      770 SH       DEFINED                 770      0    0
JOHNSON & JOHNSON                 COM               478160104    2,852   46,842 SH       SOLE                 46,842      0    0
JOHNSON & JOHNSON                 COM               478160104      552    9,060 SH       DEFINED               9,060      0    0
KIMBERLY CLARK CORP               COM               494368103      272    4,619 SH       SOLE                  4,619      0    0
KIMBERLY CLARK CORP               COM               494368103      112    1,898 SH       DEFINED               1,498     40    0
KRAFT FOODS INC                   CL A              50075N104      345   13,117 SH       SOLE                 13,117      0    0
KRAFT FOODS INC                   CL A              50075N104       52    1,961 SH       DEFINED               1,961      0    0
LOCKHEED MARTIN CORP              COM               539830109      178    2,280 SH       SOLE                  2,280      0    0
LOCKHEED MARTIN CORP              COM               539830109       62      800 SH       DEFINED                 800      0    0
LOWES COS INC                     COM               548661107       60    2,850 SH       SOLE                  2,850      0    0
LOWES COS INC                     COM               548661107      300   14,350 SH       DEFINED              14,350      0    0
MANULIFE FINL CORP                COM               56501R106      209    9,988 SH       SOLE                  9,988      0    0
MANULIFE FINL CORP                COM               56501R106      452   21,608 SH       DEFINED              21,608      0    0
MARRIOTT INTL INC NEW             CL A              571903202      264    9,556 SH       SOLE                  9,556      0    0
MARRIOTT INTL INC NEW             CL A              571903202       31    1,107 SH       DEFINED               1,107      0    0
MCDONALDS CORP                    COM               580135101      177    3,104 SH       SOLE                  3,104      0    0
MCDONALDS CORP                    COM               580135101       71    1,250 SH       DEFINED               1,250      0    0
MEDCO HEALTH SOLUTIONS INC        COM               58405U102      282    5,100 SH       SOLE                  5,100      0    0
MEDCO HEALTH SOLUTIONS INC        COM               58405U102       79    1,422 SH       DEFINED               1,422      0    0
MEDTRONIC INC                     COM               585055106      260    7,054 SH       SOLE                  7,054      0    0
MEDTRONIC INC                     COM               585055106      105    2,850 SH       DEFINED               2,850      0    0
MERCK & CO INC                    COM               589331107      482   15,227 SH       SOLE                 15,227      0    0
MERCK & CO INC                    COM               589331107      221    6,975 SH       DEFINED               6,975      0    0
MICROSOFT CORP                    COM               594918104      331   12,874 SH       SOLE                 12,874      0    0
MICROSOFT CORP                    COM               594918104      806   31,325 SH       DEFINED              31,325      0    0
NORFOLK SOUTHERN CORP             COM               655844108      570   13,219 SH       SOLE                 13,219      0    0






                                                  FORM 13F INFORMATION TABLE

            COLUMN 1                  COLUMN 2      COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
--------------------------------- ----------------- --------- -------- ----------------- ---------- -------- -------------------
                                                               VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
         NAME OF ISSUER            TITLE OF CLASS     CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
--------------------------------- ----------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                           
NORFOLK SOUTHERN CORP             COM               655844108      319    7,400 SH       DEFINED               7,400      0    0
ORACLE CORP                       COM               68389X105       57    2,750 SH       SOLE                  2,750      0    0
ORACLE CORP                       COM               68389X105      357   17,130 SH       DEFINED              17,130      0    0
PEPSICO INC                       COM               713448108      568    9,675 SH       SOLE                  9,675      0    0
PEPSICO INC                       COM               713448108      908   15,473 SH       DEFINED              15,473      0    0
PFIZER INC                        COM               717081103      777   46,924 SH       SOLE                 46,924      0    0
PFIZER INC                        COM               717081103      995   60,121 SH       DEFINED              60,121      0    0
PHILIP MORRIS INTL INC            COM               718172109      943   19,350 SH       SOLE                 19,350      0    0
PHILIP MORRIS INTL INC            COM               718172109      294    6,042 SH       DEFINED               6,042      0    0
PROCTER & GAMBLE CO               COM               742718109      585   10,100 SH       SOLE                 10,100      0    0
PROCTER & GAMBLE CO               COM               742718109      234    4,041 SH       DEFINED               4,041      0    0
PROTECTIVE LIFE CORP              COM               743674103    4,980  232,488 SH       SOLE                232,488      0    0
QWEST COMMUNICATIONS INTL IN      COM               749121109        1      361 SH       SOLE                    361      0    0
QWEST COMMUNICATIONS INTL IN      COM               749121109       45   11,699 SH       DEFINED              11,699      0    0
REGIONS FINANCIAL CORP NEW        COM               7591EP100      504   81,091 SH       SOLE                 81,091      0    0
REGIONS FINANCIAL CORP NEW        COM               7591EP100       17    2,710 SH       DEFINED               2,710      0    0
ROYAL DUTCH SHELL PLC             SPONS ADR A       780259206      307    5,370 SH       DEFINED               5,370      0    0
SARA LEE CORP                     COM               803111103      100    8,985 SH       SOLE                  8,985      0    0
SARA LEE CORP                     COM               803111103       92    8,300 SH       DEFINED               8,300      0    0
SCHERING PLOUGH CORP              COM               806605101       23      800 SH       SOLE                    800      0    0
SCHERING PLOUGH CORP              COM               806605101      468   16,570 SH       DEFINED              16,570      0    0
SCHLUMBERGER LTD                  COM               806857108      226    3,793 SH       SOLE                  3,793      0    0
SCHLUMBERGER LTD                  COM               806857108      470    7,890 SH       DEFINED               7,890      0    0
SPACE PROPULSION SYS INC          COM               846192102        0   16,250 SH       SOLE                 16,250      0    0
TJX COS INC NEW                   COM               872540109       10      275 SH       SOLE                    275      0    0
TJX COS INC NEW                   COM               872540109      193    5,200 SH       DEFINED               5,200      0    0
TARGET CORP                       COM               87612E106       62    1,326 SH       SOLE                  1,326      0    0
TARGET CORP                       COM               87612E106      243    5,210 SH       DEFINED               5,210      0    0
TEXAS INSTRS INC                  COM               882508104      127    5,342 SH       SOLE                  5,342      0    0
TEXAS INSTRS INC                  COM               882508104      180    7,607 SH       DEFINED               7,607      0    0
3M CO                             COM               88579Y101      504    6,833 SH       SOLE                  6,833      0    0
3M CO                             COM               88579Y101      248    3,358 SH       DEFINED               3,358      0    0
TRAVELERS COMPANIES INC           COM               89417E109        2       45 SH       SOLE                     45      0    0
TRAVELERS COMPANIES INC           COM               89417E109      218    4,421 SH       DEFINED               4,421      0    0
UNITED TECHNOLOGIES CORP          COM               913017109      203    3,330 SH       SOLE                  3,330      0    0
UNITED TECHNOLOGIES CORP          COM               913017109      558    9,155 SH       DEFINED               9,155      0    0
VERIZON COMMUNICATIONS INC        COM               92343V104      622   20,551 SH       SOLE                 20,551      0    0
VERIZON COMMUNICATIONS INC        COM               92343V104      727   24,015 SH       DEFINED              24,015      0    0
WAL MART STORES INC               COM               931142103      863   17,586 SH       SOLE                 17,586      0    0
WAL MART STORES INC               COM               931142103      400    8,150 SH       DEFINED               8,150      0    0
WELLPOINT INC                     COM               94973V107      256    5,400 SH       DEFINED               5,400      0    0
WELLS FARGO & CO NEW              COM               949746101      390   13,826 SH       SOLE                 13,826      0    0
WELLS FARGO & CO NEW              COM               949746101      211    7,478 SH       DEFINED               7,478      0    0
WYETH                             COM               983024100      651   13,396 SH       SOLE                 13,396      0    0
WYETH                             COM               983024100      835   17,179 SH       DEFINED              17,179      0    0