UNITED STATES
                       SECURITIES AND EXCHANGE COMMISSION
                             Washington, D.C. 20549

                                    Form 13F
                               Form 13F COVER PAGE
Report for the Calendar Year or Quarter Ended: 12/31/2009
Check here if Amendment []; Amendment Number:
                                               -------
This Amendment (Check only one.):               [] is a restatement.
                                                [] adds new holdings entries.

Institutional Investment Manager Filing this Report:


Name:      Burke & Herbert Bank & Trust Co.

Address:   PO Box 268 Alexandria, VA 22314


Form 13F File Number: 28-05313


The institutional investment manager filing this report and the person by whom
it is signed hereby represent that the person signing the report is authorized
to submit it, that all information contained herein is true, correct and
complete, and that it is understood that all required items, statements,
schedules, lists, and tables, are considered integral parts of this form.

Persons Signing this Report on Behalf of Reporting Manager:

Name:   Judith A. Cagnon
Title:  Vice President
Phone:  703-549-6600

Signature,  Place,  and  Date  of  Signing:

/s/ Judith A. Cagnon               Alexandria, VA                     1/8/2010
---------------------------------  ---------------------------------  ----------
[Signature]                        [City, State]                      [Date]



Report  Type  (Check  only  one):

[X]     13F HOLDINGS REPORT. (Check here if all holdings of this reporting
        manager are reported in this report.)

[ ]     13F NOTICE. (Check here if no holdings reported are in this report,
        and all holdings are reported by other reporting manager(s).)

[ ]     13F COMBINATION REPORT. (Check here if a portion of the holdings for
        this reporting manager are reported in this report and a portion are
        reported by other reporting manager(s).)

List of Other Managers Reporting for this Manager:




                              Form 13F SUMMARY PAGE

Report Summary:

Number of Other Included Managers:                    0

Form 13F Information Table Entry Total:             166

Form 13F Information Table Value Total:  $       82,221
                                         --------------
                                         (In Thousands)


List of Other Included Managers:

Provide a numbered list of the name(s) and 13F file number(s) of all
institutional investment managers with respect to which this report is filed,
other than the manager filing this report.
NONE







                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
TRANSOCEAN LTD               REG SHS        H8817H100       36      440 SH       SOLE                    440      0    0
TRANSOCEAN LTD               REG SHS        H8817H100      299    3,613 SH       DEFINED               3,613      0    0
AT&T INC                     COM            00206R102      800   28,548 SH       SOLE                 28,548      0    0
AT&T INC                     COM            00206R102      787   28,061 SH       DEFINED              28,061      0    0
ABBOTT LABS                  COM            002824100      626   11,600 SH       SOLE                 11,600      0    0
ABBOTT LABS                  COM            002824100      446    8,260 SH       DEFINED               8,260      0    0
AIR PRODS & CHEMS INC        COM            009158106       24      300 SH       SOLE                    300      0    0
AIR PRODS & CHEMS INC        COM            009158106      190    2,350 SH       DEFINED               2,350      0    0
ALLIED CAP CORP NEW          COM            01903Q108        0       25 SH       SOLE                     25      0    0
ALLIED CAP CORP NEW          COM            01903Q108       47   12,975 SH       DEFINED              12,975      0    0
ALTRIA GROUP INC             COM            02209S103      387   19,700 SH       SOLE                 19,700      0    0
ALTRIA GROUP INC             COM            02209S103      114    5,800 SH       DEFINED               5,800      0    0
AMERICAN EXPRESS CO          COM            025816109      201    4,966 SH       SOLE                  4,966      0    0
AMERICAN EXPRESS CO          COM            025816109       51    1,250 SH       DEFINED               1,250      0    0
APPLE INC                    COM            037833100      166      790 SH       SOLE                    790      0    0
APPLE INC                    COM            037833100      342    1,625 SH       DEFINED               1,625      0    0
AUTOMATIC DATA PROCESSI      COM            053015103       13      300 SH       SOLE                    300      0    0
AUTOMATIC DATA PROCESSI      COM            053015103      283    6,600 SH       DEFINED               6,600      0    0
BP PLC                       SPON ADR       055622104      831   14,332 SH       SOLE                 14,332      0    0
BP PLC                       SPON ADR       055622104      284    4,897 SH       DEFINED               4,897      0    0
BANCO BILBAO VIZCAYA AR      SPON ADR       05946K101      253   14,045 SH       SOLE                 14,045      0    0
BANK OF AMERICA CORPORA      COM            060505104      117    7,758 SH       SOLE                  7,758      0    0
BANK OF AMERICA CORPORA      COM            060505104      541   35,896 SH       DEFINED              35,896      0    0
BANK OF NEW YORK MELLON      COM            064058100        4      150 SH       SOLE                    150      0    0
BANK OF NEW YORK MELLON      COM            064058100      423   15,139 SH       DEFINED              15,139      0    0
BAXTER INTL INC              COM            071813109      197    3,350 SH       SOLE                  3,350      0    0
BAXTER INTL INC              COM            071813109       44      750 SH       DEFINED                 750      0    0
BERKSHIRE HATHAWAY INC       CL A           084670108      298        3 SH       SOLE                      3      0    0
BLACKROCK INC                COM            09247X101       99      425 SH       SOLE                    425      0    0
BLACKROCK INC                COM            09247X101      197      850 SH       DEFINED                 850      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      251    9,926 SH       SOLE                  9,926      0    0
BRISTOL MYERS SQUIBB CO      COM            110122108      152    6,020 SH       DEFINED               6,020      0    0
BUCKEYE PARTNERS L P         UNIT LTD PARTN 118230101      218    4,000 SH       DEFINED               4,000      0    0
BURKE&HERBERT B&T ALEXA      COM            121331102   12,176    6,727 SH       SOLE                  6,727      0    0
BURKE&HERBERT B&T ALEXA      COM            121331102    1,482      819 SH       DEFINED                 819      0    0
BURLINGTON NORTHN SANTA      COM            12189T104       32      329 SH       SOLE                    329      0    0
BURLINGTON NORTHN SANTA      COM            12189T104      398    4,038 SH       DEFINED               4,038      0    0
CIGNA CORP                   COM            125509109      535   15,180 SH       DEFINED              15,180      0    0
CVS CAREMARK CORPORATIO      COM            126650100      282    8,750 SH       DEFINED               8,750      0    0
CHEVRON CORP NEW             COM            166764100      828   10,749 SH       SOLE                 10,749      0    0
CHEVRON CORP NEW             COM            166764100    1,638   21,271 SH       DEFINED              21,271      0    0
CISCO SYS INC                COM            17275R102      336   14,024 SH       SOLE                 14,024      0    0
CISCO SYS INC                COM            17275R102      189    7,900 SH       DEFINED               7,900      0    0
CITIGROUP INC                COM            172967101       10    2,903 SH       SOLE                  2,903      0    0
CITIGROUP INC                COM            172967101      300   90,695 SH       DEFINED              90,695      0    0
COCA COLA CO                 COM            191216100      671   11,780 SH       SOLE                 11,780      0    0
COCA COLA CO                 COM            191216100      539    9,450 SH       DEFINED               9,450      0    0
COLGATE PALMOLIVE CO         COM            194162103      271    3,295 SH       SOLE                  3,295      0    0
COLGATE PALMOLIVE CO         COM            194162103      160    1,950 SH       DEFINED               1,950      0    0
CONOCOPHILLIPS               COM            20825C104       30      580 SH       SOLE                    580      0    0
CONOCOPHILLIPS               COM            20825C104      224    4,380 SH       DEFINED               4,380      0    0
CONSOLIDATED EDISON INC      COM            209115104       94    2,071 SH       SOLE                  2,071      0    0
CONSOLIDATED EDISON INC      COM            209115104      131    2,879 SH       DEFINED               2,879      0    0
DELL INC                     COM            24702R101       62    4,350 SH       SOLE                  4,350      0    0
DELL INC                     COM            24702R101      118    8,200 SH       DEFINED               8,200      0    0
DISNEY WALT CO               COM            254687106      245    7,592 SH       SOLE                  7,592      0    0
DISNEY WALT CO               COM            254687106      440   13,650 SH       DEFINED              13,650      0    0
DOMINION RES INC VA NEW      COM            25746U109      422   10,842 SH       SOLE                 10,842      0    0
DOMINION RES INC VA NEW      COM            25746U109      433   11,124 SH       DEFINED              11,124      0    0
DU PONT E I DE NEMOURS       COM            263534109      433   12,872 SH       SOLE                 12,872      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
DU PONT E I DE NEMOURS       COM            263534109      570   16,926 SH       DEFINED              16,926      0    0
DUKE ENERGY CORP NEW         COM            26441C105       30    1,763 SH       SOLE                  1,763      0    0
DUKE ENERGY CORP NEW         COM            26441C105      184   10,700 SH       DEFINED              10,700      0    0
DUN & BRADSTREET CORP D      COM            26483E100      304    3,600 SH       DEFINED               3,600      0    0
EMERSON ELEC CO              COM            291011104      143    3,364 SH       SOLE                  3,364      0    0
EMERSON ELEC CO              COM            291011104      101    2,360 SH       DEFINED               2,360      0    0
EXELON CORP                  COM            30161N101      141    2,882 SH       SOLE                  2,882      0    0
EXELON CORP                  COM            30161N101      186    3,800 SH       DEFINED               3,800      0    0
EXXON MOBIL CORP             COM            30231G102    2,982   43,734 SH       SOLE                 43,734      0    0
EXXON MOBIL CORP             COM            30231G102    4,555   66,800 SH       DEFINED              66,800      0    0
FPL GROUP INC                COM            302571104      266    5,032 SH       SOLE                  5,032      0    0
FPL GROUP INC                COM            302571104      158    2,990 SH       DEFINED               2,990      0    0
FEDERAL REALTY INVT TR       SH BEN INT NEW 313747206      620    9,150 SH       DEFINED               9,150      0    0
FLUOR CORP NEW               COM            343412102      279    6,200 SH       DEFINED               6,200      0    0
FORTUNE BRANDS INC           COM            349631101      324    7,500 SH       DEFINED               7,500      0    0
FREEPORT-MCMORAN COPPER      COM            35671D857      917   11,421 SH       DEFINED              11,421      0    0
GENERAL ELECTRIC CO          COM            369604103    1,593  105,291 SH       SOLE                105,291      0    0
GENERAL ELECTRIC CO          COM            369604103    1,778  117,523 SH       DEFINED             117,523      0    0
GLAXOSMITHKLINE PLC          SPONS ADR      37733W105      144    3,416 SH       SOLE                  3,416      0    0
GLAXOSMITHKLINE PLC          SPONS ADR      37733W105       67    1,582 SH       DEFINED               1,582      0    0
GOLDMAN SACHS GROUP INC      COM            38141G104       77      455 SH       SOLE                    455      0    0
GOLDMAN SACHS GROUP INC      COM            38141G104      132      780 SH       DEFINED                 780      0    0
GOOGLE INC                   CL A           38259P508       19       30 SH       SOLE                     30      0    0
GOOGLE INC                   CL A           38259P508      211      340 SH       DEFINED                 340      0    0
HARRIS CORP DEL              COM            413875105      105    2,200 SH       SOLE                  2,200      0    0
HARRIS CORP DEL              COM            413875105      119    2,500 SH       DEFINED               2,500      0    0
HEWLETT PACKARD CO           COM            428236103    1,072   20,812 SH       SOLE                 20,812      0    0
HEWLETT PACKARD CO           COM            428236103      940   18,245 SH       DEFINED              18,245      0    0
HOME DEPOT INC               COM            437076102      138    4,775 SH       SOLE                  4,775      0    0
HOME DEPOT INC               COM            437076102      164    5,683 SH       DEFINED               5,683      0    0
HONEYWELL INTL INC           COM            438516106      206    5,262 SH       SOLE                  5,262      0    0
HONEYWELL INTL INC           COM            438516106      192    4,900 SH       DEFINED               4,900      0    0
INTEL CORP                   COM            458140100      342   16,778 SH       SOLE                 16,778      0    0
INTEL CORP                   COM            458140100      170    8,325 SH       DEFINED               8,325      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      708    5,412 SH       SOLE                  5,412      0    0
INTERNATIONAL BUSINESS MACHS COM            459200101      671    5,127 SH       DEFINED               5,127      0    0
ISHARES TR INDEX             BARCLY USAGG B 464287226      244    2,365 SH       SOLE                  2,365      0    0
ISHARES TR INDEX             RUSSELL 3000   464287689      213    3,270 SH       DEFINED               3,270      0    0
JPMORGAN CHASE & CO          COM            46625H100      184    4,405 SH       SOLE                  4,405      0    0
JPMORGAN CHASE & CO          COM            46625H100       32      770 SH       DEFINED                 770      0    0
JOHNSON & JOHNSON            COM            478160104    3,017   46,842 SH       SOLE                 46,842      0    0
JOHNSON & JOHNSON            COM            478160104      596    9,260 SH       DEFINED               9,260      0    0
KIMBERLY CLARK CORP          COM            494368103      294    4,619 SH       SOLE                  4,619      0    0
KIMBERLY CLARK CORP          COM            494368103      121    1,898 SH       DEFINED               1,498    400    0
KRAFT FOODS INC              CL A           50075N104      357   13,117 SH       SOLE                 13,117      0    0
KRAFT FOODS INC              CL A           50075N104       53    1,961 SH       DEFINED               1,961      0    0
LOCKHEED MARTIN CORP         COM            539830109      172    2,280 SH       SOLE                  2,280      0    0
LOCKHEED MARTIN CORP         COM            539830109       45      600 SH       DEFINED                 600      0    0
LOWES COS INC                COM            548661107       69    2,950 SH       SOLE                  2,950      0    0
LOWES COS INC                COM            548661107      336   14,350 SH       DEFINED              14,350      0    0
MANULIFE FINL CORP           COM            56501R106      183    9,988 SH       SOLE                  9,988      0    0
MANULIFE FINL CORP           COM            56501R106      396   21,608 SH       DEFINED              21,608      0    0
MARRIOTT INTL INC NEW        CL A           571903202      261    9,588 SH       SOLE                  9,588      0    0
MARRIOTT INTL INC NEW        CL A           571903202       30    1,110 SH       DEFINED               1,110      0    0
MCDONALDS CORP               COM            580135101      203    3,254 SH       SOLE                  3,254      0    0
MCDONALDS CORP               COM            580135101       78    1,250 SH       DEFINED               1,250      0    0
MEDCO HEALTH SOLUTIONS       COM            58405U102      320    5,000 SH       SOLE                  5,000      0    0
MEDCO HEALTH SOLUTIONS       COM            58405U102       91    1,422 SH       DEFINED               1,422      0    0
MEDTRONIC INC                COM            585055106      310    7,054 SH       SOLE                  7,054      0    0
MEDTRONIC INC                COM            585055106      132    3,000 SH       DEFINED               3,000      0    0






                                                  FORM 13F INFORMATION TABLE

          COLUMN 1              COLUMN 2    COLUMN 3  COLUMN 4     COLUMN 5       COLUMN 6  COLUMN 7      COLUMN 8
---------------------------- -------------- --------- -------- ----------------- ---------- -------- -------------------
                                                       VALUE   SHRS OR  SH/ PUT/ INVESTMENT  OTHER    VOTING AUTHORITY
       NAME OF ISSUER        TITLE OF CLASS   CUSIP   (x$1000) PRN AMT  PRN CALL DISCRETION MANAGERS  SOLE   SHARED NONE
---------------------------- -------------- --------- -------- -------- --- ---- ---------- -------- ------- ------ ----
                                                                                   
MERCK & CO INC NEW           COM            58933Y105      581   15,888 SH       SOLE                 15,888      0    0
MERCK & CO INC NEW           COM            58933Y105      604   16,530 SH       DEFINED              16,530      0    0
MICROSOFT CORP               COM            594918104      389   12,759 SH       SOLE                 12,759      0    0
MICROSOFT CORP               COM            594918104      943   30,925 SH       DEFINED              30,925      0    0
NORFOLK SOUTHERN CORP        COM            655844108      701   13,369 SH       SOLE                 13,369      0    0
NORFOLK SOUTHERN CORP        COM            655844108      383    7,300 SH       DEFINED               7,300      0    0
ORACLE CORP                  COM            68389X105       72    2,950 SH       SOLE                  2,950      0    0
ORACLE CORP                  COM            68389X105      408   16,630 SH       DEFINED              16,630      0    0
PEPSICO INC                  COM            713448108      588    9,675 SH       SOLE                  9,675      0    0
PEPSICO INC                  COM            713448108      941   15,473 SH       DEFINED              15,473      0    0
PFIZER INC                   COM            717081103    1,093   60,114 SH       SOLE                 60,114      0    0
PFIZER INC                   COM            717081103    1,418   77,939 SH       DEFINED              77,939      0    0
PHILIP MORRIS INTL INC       COM            718172109      932   19,350 SH       SOLE                 19,350      0    0
PHILIP MORRIS INTL INC       COM            718172109      291    6,042 SH       DEFINED               6,042      0    0
PROCTER & GAMBLE CO          COM            742718109      615   10,150 SH       SOLE                 10,150      0    0
PROCTER & GAMBLE CO          COM            742718109      427    7,041 SH       DEFINED               7,041      0    0
PROTECTIVE LIFE CORP         COM            743674103    3,848  232,488 SH       SOLE                232,488      0    0
QWEST COMMUNICATIONS IN      COM            749121109        2      361 SH       SOLE                    361      0    0
QWEST COMMUNICATIONS IN      COM            749121109       49   11,550 SH       DEFINED              11,550      0    0
REGIONS FINANCIAL CORP       NEW   COM      7591EP100      429   81,091 SH       SOLE                 81,091      0    0
REGIONS FINANCIAL CORP       NEW   COM      7591EP100       14    2,710 SH       DEFINED               2,710      0    0
ROYAL DUTCH SHELL PLC        SPONS ADR A    780259206      323    5,370 SH       SOLE                  5,370      0    0
SARA LEE CORP                COM            803111103      102    8,385 SH       SOLE                  8,385      0    0
SARA LEE CORP                COM            803111103      101    8,300 SH       DEFINED               8,300      0    0
SCHLUMBERGER LTD             COM            806857108      253    3,893 SH       SOLE                  3,893      0    0
SCHLUMBERGER LTD             COM            806857108      514    7,890 SH       DEFINED               7,890      0    0
SPACE PROPULSION SYS IN      COM            846192102        1   16,250 SH       SOLE                 16,250      0    0
TJX COS INC NEW              COM            872540109       10      275 SH       SOLE                    275      0    0
TJX COS INC NEW              COM            872540109      190    5,200 SH       DEFINED               5,200      0    0
TARGET CORP                  COM            87612E106       64    1,326 SH       SOLE                  1,326      0    0
TARGET CORP                  COM            87612E106      252    5,210 SH       DEFINED               5,210      0    0
TEXAS INSTRS INC             COM            882508104      139    5,342 SH       SOLE                  5,342      0    0
TEXAS INSTRS INC             COM            882508104      198    7,607 SH       DEFINED               7,607      0    0
3M CO                        COM            88579Y101      563    6,813 SH       SOLE                  6,813      0    0
3M CO                        COM            88579Y101      278    3,358 SH       DEFINED               3,358      0    0
TRAVELERS COMPANIES INC      COM            89417E109        2       45 SH       SOLE                     45      0    0
TRAVELERS COMPANIES INC      COM            89417E109      220    4,421 SH       DEFINED               4,421      0    0
UNITED TECHNOLOGIES COR      COM            913017109      231    3,330 SH       SOLE                  3,330      0    0
UNITED TECHNOLOGIES COR      COM            913017109      635    9,155 SH       DEFINED               9,155      0    0
VERIZON COMMUNICATIONS       COM            92343V104      681   20,551 SH       SOLE                 20,551      0    0
VERIZON COMMUNICATIONS       COM            92343V104      902   27,215 SH       DEFINED              27,215      0    0
WAL MART STORES INC          COM            931142103      943   17,636 SH       SOLE                 17,636      0    0
WAL MART STORES INC          COM            931142103      436    8,150 SH       DEFINED               8,150      0    0
WELLPOINT INC                COM            94973V107      315    5,400 SH       DEFINED               5,400      0    0
WELLS FARGO & CO NEW         COM            949746101      393   14,576 SH       SOLE                 14,576      0    0
WELLS FARGO & CO NEW         COM            949746101      202    7,478 SH       DEFINED               7,478      0    0